File:Single YFP molecule superresolution microscopy.png
Summary
Superresolution Microscopy/ Optical nanoscopy using SPDMphymod as a new localization microscopy technique for standard fluorescent dyes
SPDMphymod image of protein distribution in a human cancer cell. Enlarged inserts above and below: Positions of single fluorescent molecules in Gaussian intensity shape of 5 nm standard deviation. Typical distance measurements in the 15 nm range are highlighted (<a href="//commons.wikimedia.org/w/index.php?title=Christoph_Cremer&action=edit&redlink=1" class="new" title="Christoph Cremer (page does not exist)">Christoph Cremer</a> lab/KIP, University of Heidelberg.
Fundamental to SPDMphymod are blinking phenomena (flashes of fluorescence), induced by reversible bleaches (metastable dark states). Individual molecules of the same spectral emission color can be detected. Counting individual molecules up to a density of 1000/µm2 – at present, this is possible in an area of up to 5000 µm2.
Basic publications:
Lemmer P, Gunkel M, Baddeley D, Kaufmann R, Urich A, Weiland Y, Reymann J, Müller P, Hausmann M, Cremer C: SPDM – Light Microscopy with Single Molecule Resolution at the Nanoscale. In: Applied Physics B 2008; 93: 1–12
Manuel Gunkel, Fabian Erdel, Karsten Rippe, Paul Lemmer, Rainer Kaufmann, Christoph Hörmann, Roman Amberger and Christoph Cremer: Dual color localization microscopy of cellular nanostructures. In: Biotechnology Journal, 2009, 4, 927-938. ISSN 1860-6768
Licensing
Lua error in package.lua at line 80: module 'strict' not found.
File history
Click on a date/time to view the file as it appeared at that time.
| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 07:05, 6 January 2017 | | 340 × 686 (66 KB) | 127.0.0.1 (talk) | Superresolution Microscopy/ Optical nanoscopy using SPDMphymod as a new localization microscopy technique for standard fluorescent dyes <p>SPDMphymod image of protein distribution in a human cancer cell. Enlarged inserts above and below: Positions of single fluorescent molecules in Gaussian intensity shape of 5 nm standard deviation. Typical distance measurements in the 15 nm range are highlighted (<a href="//commons.wikimedia.org/w/index.php?title=Christoph_Cremer&action=edit&redlink=1" class="new" title="Christoph Cremer (page does not exist)">Christoph Cremer</a> lab/KIP, University of Heidelberg. </p> <p>Fundamental to SPDMphymod are blinking phenomena (flashes of fluorescence), induced by reversible bleaches (metastable dark states). Individual molecules of the same spectral emission color can be detected. Counting individual molecules up to a density of 1000/µm2 – at present, this is possible in an area of up to 5000 µm2. </p> <p>Basic publications: </p> <p>Lemmer P, Gunkel M, Baddeley D, Kaufmann R, Urich A, Weiland Y, Reymann J, Müller P, Hausmann M, Cremer C: SPDM – Light Microscopy with Single Molecule Resolution at the Nanoscale. In: Applied Physics B 2008; 93: 1–12 </p> Manuel Gunkel, Fabian Erdel, Karsten Rippe, Paul Lemmer, Rainer Kaufmann, Christoph Hörmann, Roman Amberger and Christoph Cremer: Dual color localization microscopy of cellular nanostructures. In: Biotechnology Journal, 2009, 4, 927-938. ISSN 1860-6768 |
- You cannot overwrite this file.
File usage
The following 2 pages link to this file:
