Tus (biology)
Tus, also known as terminus utilization substance, is a protein that binds to terminator sequences and acts as a counter-helicase when it comes in contact with an advancing helicase.[2] The bound Tus protein effectively halts DNA polymerase movement.[2] Tus helps end DNA replication in prokaryotes.[2]
In E. coli, Tus binds to 10 closely related sites encoded in the chromosome. These sites bind 23 base-pairs. The 10 sites are called Ter sites, and are designated TerA, TerB, ..., TerJ. These binding sites are asymmetric, such that when a Tus-Ter complex (Tus protein bound to a Ter site) is encountered by a replication fork from one direction, the complex is dissociated and replication continues (permissive). But when encountered from the other direction, the Tus-Ter complex provides a much larger kinetic barrier and halts replication (non-permissive). The multiple Ter sites in the chromosome are oriented such that the two oppositely moving replication forks are both stalled in the desired termination region.[3]
Bacillus subtilis utilize replication terminator protein (RTP) instead of Tus.
Further reading
- "Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: a model derived from DNA-binding studies of mutant proteins by surface plasmon resonance."[4]
- "Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex."[5]
- "A molecular mousetrap determines polarity of termination of DNA replication in E. coli."[3]
- "Isolation and characterization of mutants of Tus, the replication arrest protein of Escherichia coli." [6]
- "Biophysical characteristics of Tus, the replication arrest protein of Escherichia coli."[7]
- "Structure of a replication-terminator protein complexed with DNA."[1]
References
<templatestyles src="Reflist/styles.css" />
Cite error: Invalid <references> tag; parameter "group" is allowed only.
<references />, or <references group="..." /><templatestyles src="Asbox/styles.css"></templatestyles>
 |
This protein-related article is a stub. You can help Wikipedia by expanding it. |
- ↑ 1.0 1.1 Lua error in package.lua at line 80: module 'strict' not found.
- ↑ 2.0 2.1 2.2 Slonczewski, Joan, and John Watkins. Foster. Microbiology: An Evolving Science. New York: W.W. Norton &, 2009. Print.
- ↑ 3.0 3.1 Lua error in package.lua at line 80: module 'strict' not found.
- ↑ Lua error in package.lua at line 80: module 'strict' not found.
- ↑ Lua error in package.lua at line 80: module 'strict' not found.
- ↑ Lua error in package.lua at line 80: module 'strict' not found.
- ↑ Lua error in package.lua at line 80: module 'strict' not found.
