Dichlorofluorescein

Dichlorofluorescein
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Names
	IUPAC name 2',7'-dichloro-3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
Identifiers
CAS Number	76-54-0
ChEBI	CHEBI:51596
ChEMBL	ChEMBL1908059
ChemSpider	58471
Jmol 3D model	Interactive image
PubChem	64944
	InChI InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H Key: VFNKZQNIXUFLBC-UHFFFAOYSA-N ; InChI=1/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H Key: VFNKZQNIXUFLBC-UHFFFAOYAO ; InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H Key: VFNKZQNIXUFLBC-UHFFFAOYSA-N ;
	SMILES Clc5cc4c(Oc1cc(O)c(Cl)cc1C34OC(=O)c2c3cccc2)cc5O ;
Properties
Chemical formula	C20H10Cl2O5
Molar mass	401.20 g·mol−1
Vapor pressure	{{{value}}}
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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	Infobox references

Dichlorofluorescein (DCF) is an organic dye of the fluorescein family, being substituted at the 2 and 7 positions by chloride.

It is also used in the cellular antioxidant activity (CAA) assay. Dichlorofluorescin is a probe that is trapped within cells and is easily oxidized to fluorescent dichlorofluorescein. The method measures the ability of compounds to prevent the formation of DCF by 2,2'-Azobis(2-amidinopropane) dihydrochloride (ABAP)-generated peroxyl radicals in human hepatocarcinoma HepG2 cells.^[3]

References

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