Isohydric principle

The isohydric principle is the phenomenon whereby multiple acid/base pairs in solution will be in equilibrium with one another, tied together by their common reagent: the hydrogen ion and hence, the pH of solution. That is, when several buffers are present together in the same solution, they are all exposed to the same hydrogen ion activity. Hence, the pK of each buffer will dictate the ratio of the concentrations of its base and weak acid forms at the given pH, in accordance with the Henderson-Hasselbalch equation.

Any condition that changes the balance of one of the buffer systems, also changes the balance of all the others because the buffer systems actually buffer one another by shifting hydrogen ions back and forth from one to the other.

The isohydric principle has special relevance to in vivo biochemistry where multiple acid/ base pairs are in solution.