Tetrahydrofolate synthase

tetrahydrofolylpolyglutamate synthase
Identifiers
EC number	6.3.2.17
CAS number	Template:CAS
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a tetrahydrofolate synthase (EC 6.3.2.17) is an enzyme that catalyzes the chemical reaction

ATP + tetrahydropteroyl-[gamma-Glu]n + L-glutamate $\rightleftharpoons$ ADP + phosphate + tetrahydropteroyl-[gamma-Glu]n⁺1

The 3 substrates of this enzyme are ATP, [[tetrahydropteroyl-[gamma-Glu]n]], and L-glutamate, whereas its 3 products are ADP, phosphate, and [[tetrahydropteroyl-[gamma-Glu]n+1]].

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is tetrahydropteroyl-gamma-polyglutamate:L-glutamate gamma-ligase (ADP-forming). Other names in common use include folylpolyglutamate synthase, folate polyglutamate synthetase, formyltetrahydropteroyldiglutamate synthetase, N10-formyltetrahydropteroyldiglutamate synthetase, folylpoly-gamma-glutamate synthase, folylpolyglutamyl synthetase, folylpoly(gamma-glutamate) synthase, folylpolyglutamate synthetase, folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase, FPGS, tetrahydrofolylpolyglutamate synthase, tetrahydrofolate:L-glutamate gamma-ligase (ADP-forming), tetrahydropteroyl-[gamma-Glu]n:L-glutamate gamma-ligase, and (ADP-forming). This enzyme participates in folate biosynthesis.

Structural studies

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1FGS, 1JBV, 1JBW, 2GC5, 2GC6, 2GCA, and 2GCB.