SLC24A5

Solute carrier family 24 (sodium/potassium/calcium exchanger), member 5
Identifiers
Symbols	SLC24A5 ; JSX; NCKX5; OCA6; SHEP4
External IDs	OMIM: 609802 MGI: 2677271 HomoloGene: 18400 IUPHAR: 1049 GeneCards: SLC24A5 Gene
Gene ontology Molecular function • calcium, potassium:sodium antiporter activity • symporter activity Cellular component • trans-Golgi network • integral component of membrane • trans-Golgi network membrane • melanosome Biological process • ion transport • potassium ion transport • calcium ion transport • ion transmembrane transport • sodium ion transmembrane transport • negative regulation of melanin biosynthetic process • response to stimulus • transmembrane transport Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	283652	317750
Ensembl	ENSG00000188467	ENSMUSG00000035183
UniProt	Q71RS6	Q8C261
RefSeq (mRNA)	NM_205850	NM_175034
RefSeq (protein)	NP_995322	NP_778199
Location (UCSC)	Chr 15: 48.12 – 48.14 Mb	Chr 2: 125.07 – 125.09 Mb
PubMed search	[1]	[2]

Sodium/potassium/calcium exchanger 5 (NCKX5), also known as solute carrier family 24 member 5 (SLC24A5), is a protein that in humans is encoded by the SLC24A5 gene that has a major influence on natural skin colour variation. The NCKX5 protein is a member of the potassium-dependent sodium/calcium exchanger family. Sequence variation in the SLC24A5 gene, particularly a non-synonymous SNP changing the amino acid at position 111 in NCKX5 from alanine to threonine, has been associated with differences in skin pigmentation.

The SLC24A5 gene's derived threonine or Ala111Thr allele (rs1426654) has been shown to be a major factor in the light skin tone of Europeans compared to Africans, and is believed to represent as much as 25–40% of the average skin tone difference between Europeans and West Africans. It has been the subject of recent selection in Europe, and is fixed in European populations.

Gene

Location of the SLC24A5 gene in human chromosome 15.

The SLC24A5 gene, in humans, is located on the long (q) arm of chromosome 15 on position 21.1, from base pair 46,200,461 to base pair 46,221,881.

Protein

NCKX5 is 43 kDa protein that is partially localized to the trans-Golgi network in melanocytes. Removal of the NCKX5 protein disrupts melanogenesis in human and mouse melanocytes, causing a significant reduction in melanin pigment production. Site-directed mutagenesis corresponding to a non-synonymous single nucleotide polymorphism in SLC24A5 alters a residue in NCKX5 (A111T) that is important for NCKX5 sodium-calcium exchanger activity.

Effect on skin color

File:Ala111Thr allele frequency distribution0.png

Global frequency distribution of the SLC24A5 gene's ancestral Ala111 allele (yellow) and its derived Ala111Thr allele (blue).

SLC24A5 appears to have played a key role in the evolution of light skin in humans of European ancestry. The gene's function in pigmentation was discovered in zebrafish as a result of the positional cloning of the gene responsible for the "golden" variety of this common pet store fish. Evidence in the International HapMap Project database of genetic variation in human populations showed that Europeans, represented by the "CEU" population, had two primary alleles differing by only one nucleotide, changing the 111th amino acid from alanine to threonine, abbreviated "A111T".

The derived threonine allele (Ala111Thr; also known as A111T or Thr111) represented 98.7 to 100% of the alleles in European samples, while the ancestral or alanine form was found in 93 to 100% of samples of Sub-Saharan Africans, East Asians and Indigenous Americans. The variation is a SNP polymorphism rs1426654, which had been previously shown to be second among 3011 tabulated SNPs ranked as ancestry-informative markers. This single change in SLC24A5 explains between 25 and 38% of the difference in skin melanin index between peoples of West African vs. European Ancestry.

Furthermore, the European mutation is associated with the largest region of diminished genetic variation in the CEU HapMap population, suggesting the possibility that the A111T mutation may be the subject of the single largest degree of selection in human populations of European ancestry. It is theorised that selection for the derived allele is based on the need for sunlight to produce the essential nutrient vitamin D. In northerly latitudes, where there is less sun, greater requirement for body coverage due to colder climate, and frequently, diets poor in vitamin D, making lighter skin more suitable for survival. Tests for this variation have obvious application to forensic science.

It has been estimated that the threonine allele became predominant among Europeans 11,000 to 19,000 years ago.

See also

• Human skin color

External links

• SLC24A5 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH)